Journal: Scientific Reports

Article Title: Ablation of cancer cell secreted neuropeptide PTHLH/PTHrP provokes anti-tumor immunity in murine tongue squamous cell carcinoma

doi: 10.1038/s41598-026-37580-1

Figure Lengend Snippet: PTHLH Knockout induces immune activation. Ablation of cancer cell-derived PTHLH increased infiltration of cytotoxic CD8 + T cells, helper CD4 + T cells, and Caspase-3 + cells, while decreasing immunosuppressive FOXP3 + Tregs and PDL1 expression, as well as TNF-α and Ki67 + cells. Non-significant trends toward reduced expression of β3-tubulin, TGFβ1, and IFNγ were also observed ( n = 3 mice/group, * p < 0.05; ** p < 0.01).

Article Snippet: For IHC, paraffin-embedded tumor tissue was sectioned in 5 μm thickness and deparaffinized into xylene and rehydrated with sequential ethanol baths followed by antigen retrieval in citrate buffer (10 mmol/L, pH = 6) for 30 min and endogenous peroxidase blocking using H 2 O 2 for 10 min. Tissue sections were incubated overnight at 4° C with primary antibody β3-tubulin (Abcam, ab18260), Ki67 (Cell Signaling, D3B5), CD8 (Abcam, ab217344), Cleaved-Caspase 3 (Cell Signaling, 9662 S), CD4 (Abcam, ab237722), FOXP3 (Cell Signaling, 12653 S), PDL1 (Cell Signaling, 13684 S), TGFB1 (Abcam, ab215715), TNFα (Sigma, SAB452982) and INFγ (ThermoFisher, PA5-95560).

Techniques: Knock-Out, Activation Assay, Derivative Assay, Expressing

Journal: PLOS One

Article Title: A novel sialylation pathway mediated by extracellular vesicles in aggressive prostate cancer

doi: 10.1371/journal.pone.0329014

Figure Lengend Snippet: IB analysis of ST6GAL1 at lighter (left panel) and darker exposures (middle panel), CNX, TSG101, Syntenin, PDL1 and CD81 in PC3, DU145 and C4-2B TCL and sEVs isolated by IDG separation (fractions 1-5 pooled; right panel); 85 µg of TCLs and 17 µg of sEV lysates were used.

Article Snippet: Rabbit monoclonal antibodies to: β-Actin (Cell Signaling, 4970, RRID:AB_2223172) 1:1000, Tumor susceptibility gene 101 (TSG101) (Abcam, ab125011, RRID:AB_10974262) 1:1000, β3 integrin (Cell Signaling, 13166S, RRID:AB_2798136) 1:1000, Syntenin (Abcam, ab133267, RRID:AB_11160262) 1:1000, Programmed cell death ligand 1 (PDL1) (Cell Signaling, 13684, RRID: AB_2687655) 1:1000 and CD41 (Abcam, ab134131, RRID:AB_2732852) 1:1000 were used.

Techniques: Isolation

Journal: PLOS One

Article Title: A novel sialylation pathway mediated by extracellular vesicles in aggressive prostate cancer

doi: 10.1371/journal.pone.0329014

Figure Lengend Snippet: (A) IB analysis of ST6GAL1 and actin in PC3 and TRAMP-C2 TCL using 1 μg/mL (right panel) or 2 μg/mL (left panel) of ST6GAL1 antibody; 40 µg of TCLs were used. (B) IB analysis of ST6GAL1, CNX and PDL1 in TRAMP-C2, RM1 and NIH3T3 TCLs; 85 µg of TCLs were used. A lane loaded with non relevant sample is included (Non relevant).

Article Snippet: Rabbit monoclonal antibodies to: β-Actin (Cell Signaling, 4970, RRID:AB_2223172) 1:1000, Tumor susceptibility gene 101 (TSG101) (Abcam, ab125011, RRID:AB_10974262) 1:1000, β3 integrin (Cell Signaling, 13166S, RRID:AB_2798136) 1:1000, Syntenin (Abcam, ab133267, RRID:AB_11160262) 1:1000, Programmed cell death ligand 1 (PDL1) (Cell Signaling, 13684, RRID: AB_2687655) 1:1000 and CD41 (Abcam, ab134131, RRID:AB_2732852) 1:1000 were used.

Techniques: